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光荣块茎植物化学成分对人类癌细胞(Hep-G2)的抗氧化活性及抗癌研究

摘要

光荣是一种生物碱植物，含有大量的生物碱成分，如秋水仙碱和光荣碱。本研究使用Hep-G2细胞(人肝癌细胞)进行抗癌检测植物化学的从古茄块茎中提取。鉴定并收集G. superba块茎。收集的样品被遮光干燥并粉碎。对粉状样品在所用溶剂的相应温度下使用索氏仪进行溶剂萃取，循环23次，持续48小时。萃取所用溶剂为甲醇、水和石油醚。植物化学定性分析。随后定量分析了特定的植物化学物质，如皂苷和生物碱，它们具有早期文献研究中提到的抗癌特性。用DPPH法测定所分析的溶剂的抗氧化活性。在每种溶剂中，甲醇提取物具有较高的抗氧化性能。采用MTT法对具有最高抗氧化性能的溶剂进行了抗Hep-G2细胞(人肝癌细胞)的抗癌试验。 The cell death percentage of Hep-G2 cells were calculated from cell viability obtained by MTT assay. Triplicate values were obtained. From the triplicate value the mean value was determined to obtain the average value for each concentration 5 μg, 10 μg, 25 μg, 50 μg, 100 μg. The higher concentration of 100 μg has the lower viable rate and 50% inhibition of viability (IC50) was determined graphically. Death rate of cells indicated the cancer inhibition rate which is due to Hep-G2 cells failed to retain the viability. Inhibition rate of 100 μg has the higher inhibition range of 54.3%. Death rate of Hep-G2 cells may be due to various reasons like protein binding, DNA replication interaction, receptor binding inhibitors, etc. this current study shows the G. superba tubers has the potential to inhibit the growth of Hep-G2 cell.

Simon SE和Jayakumar FA

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