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细胞凋亡和抗肿瘤的研究Premna coriacea在乳腺癌细胞系和体内动物模型

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Premna是一个属的200种包括一些大型攀岩者;攀援灌木,4 - 5米高。与浅棕色灰色的树皮,茎Verbenacea属于家庭。抗增殖,凋亡诱导研究,抗炎研究和体内抗癌研究已经研究了各种方法和模型PCEE。的Premna coriacea乙酸乙酯提取物(PCEE)显示潜在cyctoxicity MCF-7细胞系。结果表明,MCF-7细胞增殖显著地抑制了PCEE IC50值560μg /毫升。标准的药物阿霉素的IC50值2.38μg /毫升和PCEE与轻微的细胞毒性对MCF-7细胞系细胞死亡的56.3%。PCEE对细胞周期的影响,研究了在MCF-7细胞系中使用流式细胞仪。细胞的百分比在不同阶段像G0 / G1和G2 / M期细胞计算。结果表明,PCEE抑制细胞G0 / G1期MCF-7细胞。 Doxorubicin arrested the G2/M phase of the cell in MCF-7 cells. Apoptotic changes in cells may be studied using AO/EB fluorescent staining. The apoptotic effect of PCEE was studied in MCF-7cell lines and the percentage of apoptotic cells were calculated. The levels of p-53, phospho-p53, Bad, phospho-Bad ,cleaved caspase 3 and cleaved PARP in MCF-7cell lines were studied by ELISA method. There was a slight decrease in p53 in doxorubicin. Lifespan of ascites tumour bearing animals induced by EAC cells was found to be increased by PCEE in 200mg/kg and 400mg/kg treatment. PCEE 400mg/kg b.wt group the lifespan was increased by 42.86% and PCEE 200mg/kg group the increase was 13.34%. The survival rate of solid tumour bearing animals was also significantly increased by PCEE treatment. Similar findings was reported in DLA solid tumor model. Similar findings was reported in DLA solid tumor model. Therefore, the effect of PCEE on the inhibition of IL-1β and TNF-α was investigated. The IL-1β and TNF-α levels in supernatants of cells treated with PCEE were significantly decreased compared with the LPS group, in a dose-dependent manner. Our research indicates that these this plants possess anticancer and anti-inflammatory activities which may be due to the presence of active phytoconstituents flavonoids and phenolic compounds. It can be concluded that the plant extracts may be a source of new compounds for anticancer and anti-inflammatory.

Ashokrao鬃毛

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