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生物活性指导分离的各种提取物抗氧化活性。

摘要

这种植物Coscinium fenestratum属sterculaceae，主要成分为生物碱和黄酮类化合物。该植物来自喀拉拉邦地区，并由喀拉拉邦大学植物系鉴定。将50g生药干燥、粉碎，用石油醚、苯、氯仿、乙醇、甲醇、氯仿水等不同溶剂连续提取。采用索氏组合萃取6 ~ 8小时。浓缩萃取物，用萃取率记录萃取物的颜色。采用还原功率法对各提取物进行抗氧化活性测定。在这种方法中，吸光度的增加表示抗氧化活性。乙醇和甲醇提取物在700nm处的最大吸光度为0.916 ~ 1.113。因此，选择这两种提取物分离活性化合物。用乙醇和甲醇进行萃取，以提取其中的成分。 The ethanolic extract was packed in column and the compounds were eluted with hexane acetone and glacial acetic acid in the ratio 75:2.0:0.5. The different fractions were collected and subjected for TLC studies. The TLC studies were carried out to find out the new compounds present in the extract. The fractions were spotted on plate with the standard compound known as berberine. The 14th fraction showed two other compounds which are not according to the berberine. Those two compounds were scraped removed and dissolved in solvents separately. Again these compounds were subjected for spectral studies and anti oxidant activity. The reducing power method was used to screen the抗氧化剂活动。这两种化合物被命名为CF1和CF2。CF2表现出比CF1更强的抗氧化活性。

Anitha S, Suresh GS, Ramaiah M, Vaidya VP

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