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利用多重PCR和培养检测伊朗成年患者主要推定牙周病原体

摘要

牙周病的治疗方法是基于对疾病根源的正确诊断。本研究采用培养和多重PCR技术检测和鉴定与伊朗患者相关的牙周病原体。从100例晚期成人牙周炎(AP)患者中提取100例龈下菌斑样本。AP样本取自每个患者牙周袋最深处。样品立即培养，用基因特异性引物进行多重PCR检测。培养法牙周病原体检出率为64%，多重PCR法检出率为65%。放线菌聚集杆菌(Aa)、牙龈卟啉单胞菌(Pg)和媒介普雷沃氏菌(Pi)分别在28%、6%和3%的病变部位存在。Aa、Pg和Pi的总存在率为27%。Aa、Pg和Pi的细菌分离率分别为30%、7%和5%。多重PCR检测Aa、Pg和Pi的联合存在。 Method was 23%. This study found that Aa is the most predominant bacterium detected by both culture and multiplex PCR. Although the detection rates were marginally similar in the two methods, the PCR technique is more preferential compared with the culture, and the data of the current study recommend the use of the molecular techniques for the detection of periodontal pathogens due to labor intensive and time consuming nature of culture technique. Also, molecular techniques may be more reliable than culture because of detection of specific target genes.

艾希拉吉*，Aliramezani A, Douraghi M, Kadkhoda Z, Amin M, Heidarzadeh S, Pakbaz

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