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利用标记辅助回交育种和田间验证提高水稻对白叶枯病的抗性

摘要

白叶枯病是制约水稻种植的主要病害之一。培育持久的抗性是对抗疾病最有效和最经济的方法。本研究将4个抗性基因(Xa4、xa5、xa13和Xa21)聚合到流行cv中。利用标记辅助回交育种方法，对马苏里和两个杂交水稻亲本PRR78和KMR3进行了研究。在人工疾病和自然疾病条件下，对不同背景的9个金字塔家族进行了评价。在金字塔上接种16株水稻黄单胞菌。从印度不同地区收集的米曲菌分离株。BF16对复发亲本具有高毒力，Mahsuri的产量损失23%，PRR78的产量损失28%，KMR3的产量损失24%。BF7 (Mahsuri毒性降低10%，KMR3毒性降低13%)和BF10 (PRR78毒性降低22%)毒性最低。在Maruteru的自然条件下，病害导致Mahsuri减产22%，PRR78减产28%，KMR3减产26%。 Under both conditions, none of the pyramids were susceptible. There were no significant differences within pyramids with respect to any character evaluated. No pyramid was susceptible. The agronomic characters showed no significant difference between parent and pyramid. Yield and its related characters were statistically significant between parents and pyramids (P< 0.05). The pyramids insulated a heavy yield loss to the tune of ~28% and pyramiding of resistance genes (two dominant and two recessive) did not show any negative effect on agronomic performance of any pyramid. These pyramids can be used directly and also as valuable source of resistance in future breeding programs.

Lalitha D. Guvvala, Pranitha Koradi, Vinay Shenoy和Lalitha S. Marella

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