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现代的细菌鉴定方法

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疾病的早期诊断是必要的,以减少疾病的幼苗阶段收获为了成长健康作物最高产量。检测细菌是植物病原菌的跟踪或植物材料,尤其是当他们发生潜伏地,不会引起症状。但标识意味着孤立,描述和命名的细菌。这是一个重要的步骤在细菌感染的诊断和分类研究。没有独家或可靠的简单方法识别病原体和疾病的原因。传统的识别方法是目测的病原体原位或体外在纯文化通过显微镜检查染色反应,殖民地字符,氧需求,生理特征生化字符和血清学方法。识别通过噬菌体技术可以被认为是传统和现代的。更少的特异性和更少的歧视在物种和应变水平的缺点是传统识别方法。近年来完全新的识别技术已经开发出来。这主要是由于分子生物学的发展,揭示微生物的结构和功能。 Knowledge of the composition and the place of structural elements in microorganism has made the real finger printing methods possible such as special serological methods like Immuno-electrophoresis, Monoclonal antibodies, Immunogold labeling. Identification by separation of bacterial components using chromatographic techniques (FAA Analysis by Gas chromatography), separation of bacterial protein (PAGE). The nucleic acid based identification methods are more important because it is purely on genetic basis. Fluorescent In situ Hybridization (FISH), developed by Christoph Lengauer, based on the DNA-DNA hybridization. RFLP based on the Southern hybridization. One of the important development in genomic studies is the discovery of PCR by Kary Mullis in 1985. The PCR based identification methods are RFLP-PCR, PFGE, RAPD,REP-PCR, AFLP, Real Time PCR and BIO-PCR at tracer level infection by the bacteria, especially important for quarantine.

Raheesa M哈提卜,印度的七弦琴和Neelamma Konanavar

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