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帕罗西汀通过抗炎和抗氧化作用减轻大鼠脑缺血/再灌注损伤

摘要

脑缺血常伴有感觉运动障碍和记忆障碍。先前的研究表明，抗抑郁药物帕罗西汀具有抗炎和抗氧化作用。在本研究中，我们研究了帕罗西汀对大鼠脑缺血/再灌注引起的神经元损伤和小胶质细胞活化的保护作用。双侧颈总动脉短暂性闭塞引起脑缺血再灌注损伤。Wistar大鼠被分为假手术组、缺血组和帕罗西汀治疗组。帕罗西汀(10 mg/kg)术后7天，每日腹腔注射1次。为了评价大鼠的空间记忆，采用Morris水迷宫试验(MWM)。采用尼氏染色法检测海马锥体神经元活力。分别使用iba1免疫染色和ELISA方法检测小胶质细胞活化和促炎细胞因子(IL-1β， TNF-α)的产生。通过测量海马组织匀浆中丙二醛(MDA)的水平来评估氧化应激。 In MWM test, paroxetine significantly enhanced learning performance in rats subjected to cerebral ischemia/reperfusion. Our findings indicated that paroxetine significantly suppressed ischemia-induced microglial activation and decreased the IL-1β and TNF-α level in the hippocampus. In addition, paroxetine inhibited lipid peroxidation and decreased MDA levels in homogenates of hippocampal tissue. These results establish that paroxetine exert a protective effect against cerebral ischemia/reperfusion-induced damage to hippocampal neurons and memory impairment in rats through anti-inflammatory and antioxidant effects.

Yazdan Naderi *

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