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优化条件下普鲁兰梗孢和韦内基枝孢生产漆酶:在纺织染料脱色中的应用

摘要

纺织染料废水处理是一项艰巨的任务。本研究旨在鉴定能够产生细胞外漆酶的真菌，在优化的条件下可以脱色纺织染料。从土壤和朽木中分别分离出普鲁兰绿梗霉和韦内克支孢霉，鉴定为产漆酶真菌。研究了最大漆酶产量的生理条件。真菌生物量与漆酶产量及其生长有关。将优化后的培养基用于几种纺织染料的脱色。A.普鲁兰菌在培养第9天漆酶活性最高，为19.18 U/ml;C. werneckii偏好半乳糖，在第12天漆酶活性最高，为1.53 U/ml，在30 Ë (C)和pH 5.0时漆酶活性最佳。两株真菌均偏爱色氨酸作为氮源，并利用24 mm和0.3 mm CuSO4接种量获得最佳漆酶产量。普鲁兰漆酶是组成型漆酶，韦内基漆酶是诱导型漆酶。 The increase or decrease in laccase activity is in direct proportion to the rate of fungal growth. Optimized culture broth from A. pullulans and C. werneckii had 1.25 and 2.03 fold increase in laccase activity respectively and they were both able to decolourize malachite green specifically i. e 73% and 35% and had least decolourizing abilities on methylene blue in the absence of inducers and after three hours of incubation. The ability of these newly isolated fungal strains to produce laccase extracellularlly and also decolourize dyes would serve as a way to eliminate industrial textile waste water.

Nelson Adedeji Ademakinwa和Femi Kayode Agboola

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