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洋葱皮提取物对洋葱和口腔上皮细胞的染色

摘要

本研究的目的是通过光学显微镜图像，揭示以洋葱皮为提取液的不同部位的颊上皮细胞作为动物细胞模型和洋葱膜作为植物细胞模型的可染色性。将干净新鲜、外皮橙黄色的洋葱皮研磨20克后，精确称重。加入100毫升纯净水，煮沸30分钟，过滤。滤液储存在+4°C的冰箱中作为染料溶液使用。从染料溶液和各种金属盐(明矾，FeSO4, CuSO4)中制备了具有单独二元组合的溶剂。将洋葱膜片和口腔上皮制剂分别放入各溶液中。将制备好的材料分别在50°C和80°C的培养箱中保温1小时，然后在室温下保温1小时，即可完成染色过程。然后将洋葱膜片和颊上皮制剂从染料溶液中去除，并用纯水冲洗。将一些膜放在载玻片上，然后用盖玻片覆盖。在显微镜下检查包括涂漆的颊上皮细胞和洋葱膜细胞在内的准备工作。 It was observed that different sides of both the buccal epithelial and onion membrane cells were positively dyed in different colors and tones in the visible parts at different temperatures and in various mordants with the onion skin extract. Thus, it was determined that the aqueous extract of onion skin has potential in staining biological preparations under the conditions that gave positive results in this study.

Nilgun Guler Kusculuo

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