基质金属蛋白酶抑制的影响肩关节的糖尿病大鼠
目的:本研究的目的是揭示影响相关的强力霉素抑制基质金属蛋白酶(MMPs)在糖尿病大鼠的肩关节。材料和方法:36成人Sprague-Dawley雄性老鼠。引入一个糖尿病,35毫克/公斤intra-peritoneally链脲霉素应用24老鼠。对照组大鼠(n = 12)收到35毫克/公斤的腹腔内注射血清生理。72 h后,强力霉素应用于糖尿病组大鼠(n = 12)和对照组(n = 6)通过老鼠orogastric管期间每天130毫克/公斤两周。糖尿病对照组老鼠(n = 12)和假针灸组大鼠(n = 6) 130毫克/公斤通过orogastric管血清生理。老鼠被牺牲了三个星期。结果组织学分析。结果:平均血葡萄糖生成面89.8±10.6研究的第一天,平均水平414.7±69.4 72 h后血液葡萄糖生成链脲霉素管理局(t测试;p < 0.01)。 Histological evaluations exposed preserved joint space and were not identified inflammation, increased vascularity and cartilage degeneration with the Haematoxylin-eosin stained. Fibrosis evaluated with Masson’s trichrome stain and type I and type III collagen proportion evaluated with Picrosirius red-stain; no differences were found (Chi-square test; p>0.01). Conclusion: Streptozotocin administration was determined to be an effective experimental model in rats to induce diabetes. Sacrification time not sufficient to reveal the changes in shoulder joint capsule due to the diabetes and the effect of doxycycline. Further studies including larger groups are needed to define the mechanism of the MMPs inhibitors in the shoulder joint in chronic diabetic rats and in the generation of new clinical applications in orthopedics. Level of evidence: Level 1, experimental study.
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